Application

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

This Anti-Myeloperoxidase Antibody, clone CLB-MPO-1/1,7.17, FITC conjugate is validated for use in Flow Cytometry for the detection of Myeloperoxidase.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myeloperoxidase (EC 1.11.2.2; UniProt P05164) is encoded by the MPO gene (Gene ID 4353) in human. Myeloperoxidase/MPO is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a 745 a.a. single chain precursor with a signal peptide sequence (a.a. 1-48), myeloperoxidase is subsequently cleaved into a light chain (a.a.165-278) and a heavy chain (a.a. 279-745). The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. MPO catalyzes hydrogen peroxide-dependent oxidations and the formation of hypochlorous acid that is central to the microbicidal activity of neutrophils.

Immunogen

Purified human MPO.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse conjugate IgG2aκ monoclonal antibody in buffer containing 20mM Tris, 150mM NaCl, pH8.0 with 1% BSA (w/v), and 0.1% sodium azide.

Quality

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 0.01 mL of this antibody detected Myeloperoxidase in human peripheral blood monocytes (PBMCs).

Specificity

This monoclonal antibody reacts with majority cell types of the myeloid lineage, including myeloblasts, neutrophils, and monocytes. It does not react with human lymphocytes, thrombocytes, or erythrocytes. 96% of all acute myelogenous leukemias (AMLs) are found positive, 100% of all acute lymphoblastic leukemias (ALLs) are found negative when stained with this monoclonal antibody.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~73/83/87 kDa calculated